Southampton demanded that referee Mike Dean not officiate their fixtures and would protest against the red card shown to defender Jan Bednarek in Manchester United’s 9-0 defeat.
Saints still do not want their games to be officiated by Lee Mason, who was the fourth official at Old Trafford.
In the infamous loss of Saints by Aston Villa, Mason was the referee.
Fellow Premier League side Arsenal will protest against the dismissal of defender David Luiz in the defeat to Wolves on Tuesday.
When he took down Anthony Martial and conceded a penalty in the 86th minute, Bednarek was the second man to be sent off for Ralph Hasenhuttl’s hand.
Initially, referee Dean only gave Manchester United a spot-kick but disqualified Bednarek after being told by video assistant referee (VAR) Graham Scott to watch replays of the incident on the pitchside display.
Poland’s center-back Bednarek, 24, said that when he left the pitch, “Martial said it’s not a foul” before the hosts went on to secure a record-equalling Premier League victory.
Stuart Armstrong’s attempt caught Matty Cash on the arm against Aston Villa on Saturday, but VAR Dean did not order on-field referee Mason to award a penalty as he determined that the ball had first deflected from the leg of the defender.
Premier League teams do not legally recommend that such referees not take over their games, although it is known that when determining match appointments, Professional Game Match Officials Limited will weigh a variety of factors.
Tuesday was made up of match officials for the round of games starting on Saturday.